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VMGII Medium (Viability-Preserving Microbiostatic Medium)

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

VMGII Medium

(Viability-Preserving Microbiostatic Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

Solution 1...............................................................................900.0mL

Solution 2...............................................................................100.0mL

Salt stock solution..................................................................100.0mL

Solution 1:

Composition per 900.0mL:

Noble agar..................................................................................... 0.1g

Preparation of Solution 1: Add agar to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring

to boiling. Cool to 45°–50°C.

Solution 2:

Composition per 100.0mL:

Charcoal, bacteriological ............................................................ 10.0g

Gelatin peptone ........................................................................... 10.0g

Meat peptone................................................................................. 1.0g

Cysteine·HCl................................................................................. 0.5g

Thioglycolic acid .......................................................................0.5mL

Preparation of Solution 2: Add components to distilled/deionized

water and bring volume to 100.0mL. Mix thoroughly.

Stock Salt Solution:

Composition per liter:

Sodium glycerophosphate......................................................... 100.0g

NaCl ............................................................................................ 10.0g

KCl................................................................................................ 4.2g

CaCl2·6H2O................................................................................... 2.4g

MgSO4·7H2O ................................................................................ 1.0g

Phenylmercuric acetate ............................................................... 0.03g

Preparation of Stock Salt Solution: Add phenylmercuric acetate

to approximately 800.0mL of distilled/deionized water. Gently heat.

Add remaining components. Bring volume to 1.0L with distilled/de

ionized water.

Preparation of Medium: To 900.0mL of cooled solution 1, add

100.0mL of solution 2 and 100.0mL of stock salt solution. Mix thor

oughly. Distribute into screw-capped tubes. Autoclave for 15 min at 15

psi pressure–121°C.

Use: For the isolation and cultivation of oral streptococci, including

Streptococcus mutans and Streptococcus sanguis, and nonspore-form

ing bacteria, including Lactobacillus species from human dental

plaque.