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VM1 Medium (DSMZ Medium 890)

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

VM1 Medium

(DSMZ Medium 890)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................ 20.0g

MgCl2·6H2O ............................................................................... 12.6g

Peptone ......................................................................................... 5.0g

Na2SO4........................................................................................ 3.24g

CaCl2·2H2O ................................................................................ 2.38g

Starch ............................................................................................ 2.0g

Yeast extract.................................................................................. 1.0g

KCl.............................................................................................. 0.56g

Sulfur, powdered........................................................................... 0.5g

NH4Cl ........................................................................................... 0.3g

K2HPO4......................................................................................... 0.2g

NaHCO3...................................................................................... 0.16g

Trace elements solution ...........................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O................................................................................ 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl.............................................................................................. 1.0g

MnSO4·2H2O................................................................................ 0.5g

CoSO4·7H2O............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O .................................................................................. 0.1g

FeSO4·7H2O.................................................................................. 0.1g

NiCl2·6H2O............................................................................... 0.025g

KAl(SO4)2·12H2O....................................................................... 0.02g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·4H2O .......................................................................... 0.01g

CuSO4·5H2O............................................................................... 0.01g

Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH

to 6.5 with KOH. Add remaining components. Add distilled/deionized

water to 1.0L. Mix thoroughly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.0 us

ing H2SO4. Fill 20.0mL medium into 100.0mL serum bottles and seal

with a rubber stopper. Add atmosphere of 80% N2 + 20% air. Autoclave

for 15 min at 15 psi pressure–121°C.

Use: For the heterotrophic cultivation of Hydrogenothermus marinus.

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VM1 Medium (DSMZ Medium 890)