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Vogel-Bonner Minimal Medium

[所属分类:培养基配方] [发布时间:2021-10-11] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

K2HPO4 ...................................................................................... 10.0g

NaNH4HPO4·H2O ........................................................................ 3.5g

Citric acetate ................................................................................. 2.0g

Glucose ......................................................................................... 2.0g

MgSO4·7H2O.........................................................................200.0mg

Biotin solution............................................................................0.1mL

Biotin Solution:

Composition per 100.0mL:

Biotin ............................................................................................ 2.5g

Preparation of Biotin Solution: Add biotin to 50% ethanol and

bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Store at

5°C.

Preparation of Medium: Add components, except biotin solution,

to distilled/deionized water and bring volume to 999.9mL. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically

add 0.1mL of sterile biotin solution. Mix thoroughly. Aseptically dis

tribute into sterile tubes or flasks.

Use: For the cultivation of Neurospora species.