Anaerobic Basal Agar with Blood

[所属分类:培养基配方] [发布时间:2019-10-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic Basal Agar with Blood
Composition per liter:
Peptic digest of animal tissue......................................................16.0g
Agar ............................................................................................12.0g
Yeast extract..................................................................................7.0g
NaCl..............................................................................................5.0g
Starch ............................................................................................1.0g
Glucose .........................................................................................1.0g
Sodium pyruvate...........................................................................1.0g
L -Arginine.....................................................................................1.0g
Sodium succinate..........................................................................0.5g
Fe 4 (P 2 O 7 )·H 2 0...............................................................................0.5g
NaHCO 3 ........................................................................................0.4g
L -Cysteine HCl............................................................................0.25g
Dithiothreitol...............................................................................0.25g
Hemin.........................................................................................5.0mg
Vitamin K...................................................................................5.0mg
Horse blood, defibrinated ......................................................100.0mL
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Preparation of Medium: Add components, except horse blood, to
distilled/deionized water and bring volume to 900.0mL. Mix thorough-
ly. Adjust pH to 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 50°–55°C. Aseptically add 100.0mL of sterile horse blood. Mix thor-
oughly. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation of anaerobic microorganisms, especially
Bacteroides species and other fastidious anaerobes.