Anaerobic Agar

[所属分类:培养基配方] [发布时间:2019-10-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic Agar
Composition per liter:
Pancreatic digest of casein..........................................................20.0g
Agar ............................................................................................15.0g
NaCl..............................................................................................5.0g
Sodium thioglycolate....................................................................2.0g
Sodium formaldehyde sulfoxylate................................................1.0g
pH 7.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Adjust pH to 7.2. Distribute into tubes until medium is 3 inches
deep. Autoclave for 20 min at 15 psi pressure–121°C.
Use: For the anaerobic cultivation of Bacillus species and Sporolacto-
bacillus species.