Anaerobic Blood Agar Base with Blood and Neomycin

[所属分类:培养基配方] [发布时间:2019-10-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobic Blood Agar Base with Blood and Neomycin
Composition per liter:
Casein enzymic hydrolysate.......................................................14.5g
Agar............................................................................................14.0g
Papaic digest of soybean meal......................................................5.0g
NaCl..............................................................................................5.0g
Growth factors..............................................................................1.5g
Sheep blood, sterile defibrinated.............................................50.0mL
Selective supplement solution.................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Neomycin sulfate.....................................................................30.0mg
Preparation of Selective Supplement Solution:  Add neomycin
sulfate to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except sheep blood and
selective supplement, to distilled/deionized water and bring volume to
940.0mL. Mix thoroughly. Adjust pH to 7.3. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 50°–55°C. Aseptically add 50.0mL of sterile
sheep blood and 10.0mL of selective supplement solution. Mix thorough-
ly. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation and cultivation of Group A and Group B strep-
tococci from throat cultures and other clinical samples.