Anaerobe Agar (BAM M11)

[所属分类:培养基配方] [发布时间:2019-10-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobe Agar
(BAM M11)
Composition per 1001.5mL:
Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................15.0g
Pancreatic digest of soybean meal................................................5.0g
NaCl..............................................................................................5.0g
L- Cysteine·HCl·H 2 O solution.....................................................5.0mL
Vitamin K 1 solution...................................................................1.0mL
Hemin solution...........................................................................0.5mL
pH 7.0 ± 0.2 at 25°C
L- Cysteine·HCl·H 2 O Solution:
Composition per 5.0mL:
L- Cysteine·HCl·H 2 O......................................................................0.4g
NaOH (1N solution)...................................................................5.0mL
Preparation of  L- Cysteine·HCl·H 2 O Solution: Add  L-
cysteine·HCl·H 2 O to 5.0mL 1N NaOH. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin ...........................................................................................1.0g
Preparation of Hemin Solution:  Add hemin to distilled/deionized
water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15
min at 15 psi pressure–121°C. Cool. Refrigerate at 4°C for storage.
Vitamin K 1 Solution:
Composition per 100.0mL :
Vitamin K 1 ....................................................................................1.0g
Ethanol, absolute......................................................................20.0mL
Preparation of Vitamin K 1 Solution: Add vitamin K 1 to
100.0mL of 95% ethanol. Mix thoroughly. Solution may require 2–3
days with intermittent shaking to completely dissolve. Filter sterilize.
Refrigerate at 4°C for storage.
Preparation of Medium: Add components, except hemin solution
and vitamin K 1 solution, to distilled/deionized water and bring volume
to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Adjust pH
to 7.0 ± 0.2 at 25°C. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C. Aseptically add 0.5mL of sterile hemin solution and
1.0mL of sterile vitamin K 1 solution. Mix thoroughly. Pour into sterile
Petri dishes or distribute into sterile tubes. Reduce medium for 24h by
incubation in an anaerobic glove box or GasPak jar prior to use.
Use : For the cultivation of anaerobic bacteria such as Brucella and
Clostridium spp.