Anaerobe Medium (LMG Medium 41)

[所属分类:培养基配方] [发布时间:2019-10-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Composition per liter:
Agar Base...............................................................................800.0mL
Solution A..............................................................................100.0mL
Solution B..............................................................................100.0mL
pH 6.9 ± 0.2 at 25°C
Agar Base:
Composition per 800.0mL:
Tryptone........................................................................................5.0g
Yeast extract..................................................................................5.0g
(NH 4 ) 2 SO 4 ....................................................................................0.5g
Sodium thioglycolate....................................................................0.5g
Agar............................................................................................. 0.5g
MgSO 4 ·7H 2 O................................................................................0.1g
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O .............................................................55.0mg
Na 2 MoO 4 ·2H 2 O ........................................................................2.4 mg
Na 2 SeO 3 ·5H 2 O........................................................................0.23 mg
Preparation of Agar Base: Add components to distilled/deionized
water and bring volume to 800.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C.
Solution A:
Composition per 100.0mL:
Glucose .......................................................................................18.0g
K 2 HPO 4 .........................................................................................7.0g
KH 2 PO 4 .........................................................................................5.5g
Preparation of Solution A: Add components to 100.0mL of dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Solution B:
Composition per 100.0mL:
NaHCO 3 ......................................................................................10.0g
Preparation of Solution B: Add components to 100.0mL of dis-
tilled/deionized water. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically add solutions A and B to the
sterile agar base. Adjust pH to 6.9. Mix thoroughly. Distribute into
sterile tubes. Incubate anaerobically under 100% CO 2 gas atmosphere.
Use: For the cultivation of anaerobic bacteria.