Anaerobe Agar

[所属分类:培养基配方] [发布时间:2019-10-11] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Anaerobe Agar
Composition per 1001.5mL:
Agar ............................................................................................20.0g
Pancreatic digest of casein..........................................................17.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................5.0g
Papaic digest of soybean meal......................................................3.0g
K 2 HPO 4 .........................................................................................2.5g
Glucose .........................................................................................2.5g
L -Cystine solution......................................................................5.0mL
Vitamin K 1 solution ...................................................................1.0mL
Hemin solution...........................................................................0.5mL
pH 7.5 ± 0.2 at 25°C
L -Cystine Solution:
Composition per 5.0mL:
L- Cystine .......................................................................................0.4g
NaOH (1N solution)...................................................................5.0mL
Preparation of  L -Cystine Solution: Add  L- cystine to 5.0mL of
NaOH solution. Mix thoroughly.
Vitamin K 1 Solution:
Composition per 100.0mL:
Vitamin K 1 ....................................................................................1.0g
Ethanol.....................................................................................99.0mL
Preparation of Vitamin K 1 Solution:  Add vitamin K 1 to 99.0mL
of absolute ethanol. Mix thoroughly. Filter sterilize.
Hemin Solution:
Composition per 100.0mL:
Hemin............................................................................................1.0g
NaOH (1N solution).................................................................20.0mL
Preparation of Hemin Solution:  Add hemin to 20.0mL of 1N
NaOH solution. Mix thoroughly. Bring volume to 100.0mL with dis-
tilled/deionized water. Autoclave for 15 min at 15 psi pressure–121°C.
Cool to 45°–50°C.
Preparation of Medium: Add components, except vitamin K 1 so-
lution and hemin solution, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to
boiling. Adjust pH to 7.5. Autoclave for 15 min at 15 psi pressure–
121°C. Cool to 45°–50°C. Aseptically add 1.0mL of sterile vitamin K 1
solution and 0.5mL of sterile hemin solution. Mix thoroughly. Pour
into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of anaerobes from cosmetic products.