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Sulfitobacter pontiacus Medium (DSMZ Medium 733)

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfitobacter pontiacus Medium

(DSMZ Medium 733)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Basal salts solution.................................................................959.0mL

Biotin solution..........................................................................10.0mL

Na-acetate solution ..................................................................10.0mL

Yeast extract peptone solution .................................................10.0mL

Magnesium calcium solution ...................................................10.0mL

Trace elements solution .............................................................1.0mL

pH 7.6 ± 0.2 at 25°C

Trace Elements Solution:

Composition per liter:

MgSO4·7H2O ................................................................................ 3.0g

Nitrilotriacetic acid ....................................................................... 1.5g

NaCl .............................................................................................. 1.0g

MnSO4·2H2O ................................................................................ 0.5g

CoSO4·7H2O ............................................................................... 0.18g

ZnSO4·7H2O ............................................................................... 0.18g

CaCl2·2H2O................................................................................... 0.1g

FeSO4·7H2O.................................................................................. 0.1g

NiCl2·6H2O............................................................................... 0.025g

KAl(SO4)2·12H2O....................................................................... 0.02g

H3BO3 ......................................................................................... 0.01g

Na2MoO4·4H2O .......................................................................... 0.01g

CuSO4·5H2O............................................................................... 0.01g

Na2SeO3·5H2O........................................................................... 0.3mg

Preparation of Trace Elements Solution: Add nitrilotriacetic

acid to 500.0mL of distilled/deionized water. Dissolve by adjusting pH

to 6.5 with KOH. Add remaining components. Add distilled/deionized

water to 1.0L. Mix thoroughly. Filter sterilize.

Basal Salts Solution:

Composition per liter:

HEPES .......................................................................................... 8.0g

K2HPO4......................................................................................... 1.0g

NH4Cl ........................................................................................... 0.5g

NaCl............................................................................................ 15.0g

Preparation of Basal Salts Solution: Add components to 1.0L of

distilled/deionized water. Adjust pH to 7.5–7.8 with NaOH. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Biotin Solution:

Composition per 10.0mL:

Biotin ............................................................................................ 0.1g

Preparation of Biotin Solution: Add biotin to 10.0mL of distilled/

deionized water. Mix thoroughly. Filter sterilize.

Magnesium Calcium Solution:

Composition per 10.0mL:

MgSO4·7H2O................................................................................ 1.0g

CaCl2·2H2O ................................................................................ 0.05g

Preparation of Magnesium Calcium Solution: Add compo

nents to 10.0mL of distilled/deionized water. Mix thoroughly. Filter

sterilize.

Na-Acetate Solution:

Composition per 10.0mL:

Na-acetate ..................................................................................... 1.6g

Preparation of Na-Acetate Solution: Add Na-acetate to 10.0mL

of distilled/deionized water. Mix thoroughly. Filter sterilize.

Yeast Extract Peptone Solution:

Composition per 10.0mL:

Yeast extract.................................................................................. 1.0g

Peptone ......................................................................................... 0.5g

Preparation of Yeast Extract Peptone Solution: Add compo

nents to 10.0mL of distilled/deionized water. Mix thoroughly. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.

Preparation of Medium: Aseptically combine 959.0mL basal salts

solution, 1.0mL trace elements solution, 10.0mL Na-acetate solution,

10.0mL magnesium calcium solution, 10.0mL yeast extract peptone

solution, and 10.0mL biotin solution. Mix thoroughly. Aseptically dis

tribute to sterile tubes or flasks.

Use: For the cultivation of Sulfitobacter pontiacus.

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Sulfitobacter pontiacus Medium (DSMZ Medium 733)