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Sulfobacillus disulfidooxidans Medium (DSMZ Medium 812)

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfobacillus disulfidooxidans Medium

(DSMZ Medium 812)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

(NH4)2SO4 .................................................................................... 3.0g

KH2PO4......................................................................................... 0.5g

MgSO4·7H2O ................................................................................ 0.5g

KCl................................................................................................ 0.1g

Ca(NO3)2·4H2O............................................................................. 0.1g

Yeast extract.................................................................................. 0.1g

Glutathione solution.................................................................10.0mL

pH 2.25 ± 0.1 at 25°C

Glutathione Solution:

Composition per 10.0mL:

Glutathione.................................................................................... 1.0g

Preparation of Glutathione Solution: Add glutathione to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components, except glutathione solu

tion, to distilled/deionized water and bring volume to 990.0mL. Mix

thoroughly. Adjust pH to 2.25. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to room temperature. Aseptically add 10.0mL sterile gluta

thione solution. Mix thoroughly. Aseptically distribute into sterile tubes

or bottles.

Use: For the cultivation of Sulfobacillus disulfidooxidans.