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Sulfite HiVeg Agar with Iron

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Plant hydrolysate......................................................................... 10.0g

Na2SO3.......................................................................................... 1.0g

Ferric citrate solution ...............................................................10.0mL

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Ferric Citrate Solution:

Composition per 10.0mL:

Ferric citrate.................................................................................. 0.5g

Preparation of Ferric Citrate Solution: Add ferric citrate to dis

tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.

Filter sterilize.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Use: For the detection of thermophilic sulfide-producing anaerobes.