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Sulfite Agar

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

Sulfite Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

Pancreatic digest of casein.......................................................... 10.0g

Na2SO3 .......................................................................................... 1.0g

Iron nails .......................................................................................... 66

pH 7.6 ± 0.2 at 25°C

Source: This medium, without iron nails, is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into screw-capped tubes in 15.0mL volumes. Add

a clean iron nail to each tube. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C until ready to inoculate.

Use: For the detection and cultivation of thermophilic anaerobes that

can produce H2S from sulfite. Sulfite reduction appears as a blackening

of the medium.