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Sulfate-Reducing Medium

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium lactate .............................................................................. 3.5g

MgSO4·7H2O................................................................................ 2.0g

Peptone ......................................................................................... 2.0g

Na2SO4.......................................................................................... 1.5g

Beef extract................................................................................... 1.0g

K2HPO4......................................................................................... 0.5g

CaCl2............................................................................................. 0.1g

Fe(NH4)2(SO4)2·6H2O solution ...............................................10.0mL

Sodium ascorbate solution.......................................................10.0mL

pH 7.5 ± 0.3 at 25°C

Fe(NH4)2(SO4)2·6H2O Solution:

Composition per 100.0mL:

Fe(NH4)2(SO4)2·6H2O ................................................................ 3.92g

Preparation of Fe(NH4)2(SO4)2·6H2O Solution: Add 3.92g of

Fe(NH4)2(SO4)2·6H2O to distilled/deionized water and bring volume

to 100.0mL. Mix thoroughly. Filter sterilize. Use freshly prepared so

lution.

Sodium Ascorbate Solution:

Composition per 100.0mL:

Sodium ascorbate........................................................................ 0.05g

Preparation of Sodium Ascorbate Solution: Add sodium ascor

bate to distilled/deionized water and bring volume to 100.0mL. Mix

thoroughly. Filter sterilize. Use freshly prepared solution.

Preparation of Medium: Add components, except sodium ascor

bate solution and Fe(NH4)2(SO4)2·6H2O solution , to distilled/deionized

water and bring volume to 980.0mL. Mix thoroughly. Distribute into

screw-capped tubes in 10.0mL volumes. Autoclave for 15 min at 15 psi

pressure–121°C. Tubes must be filled to capacity after inoculation, so

prepare extra medium and sterilize in a screw-capped flask or bottle. Pri

or to inoculation, aseptically add 0.1mL of freshly prepared sterile

Fe(NH4)2(SO4)2·6H2O solution for each 10.0mL of medium in the tubes.

Also aseptically add 0.1mL of freshly prepared sterile sodium ascorbate

solution for each 10.0mL of medium in the tubes. Inoculate tubes. Fill

tubes to capacity with extra sterile medium. Screw caps tight.

Use: For the isolation, cultivation, and enumeration of iron and sulfur

bacteria.