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Sulfate-Reducing HiVeg Medium

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Part A .....................................................................................890.0mL

Part B .....................................................................................100.0mL

Part C .......................................................................................10.0mL

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from Hi

Media.

Part A:

Composition per 890.0mL:

Plant peptone................................................................................. 2.0g

MgSO4·7H2O................................................................................ 2.0g

Na2SO4.......................................................................................... 1.5g

Plant extract .................................................................................. 1.0g

K2HPO4........................................................................................... 0.5

CaCl2............................................................................................. 0.1g

Preparation of Part A: Add components to distilled/deionized wa

ter and bring volume to 890.0mL. Gently heat and bring to boiling. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Part B:

Composition per 100.0mL:

Ferrous (NH4)2SO4 ................................................................... 0.392g

Sodium ascorbate.......................................................................... 0.1g

Preparation of Part B: Add components to distilled/deionized wa

ter and bring volume to 100.0mL. Gently heat and bring to boiling. Mix

thoroughly. Filter sterilize. Prepare on day of use.

Part C:

Composition per 100.0mL:

Sodium lactate .............................................................................. 3.5g

Preparation of Part C: Add sodium lactate to distilled/deionized

water and bring volume to 100.0mL. Gently heat and bring to boiling.

Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C.

Preparation of Medium: Aseptically combine 890.0mL part A,

100.0mL part B, and 10.0mL part C. Mix thoroughly. Distribute into

screw-cap tubes. Completely fill the tubes.

Use: For the enumeration of sulfate reducing bacteria in water sam

ples.