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Sulfate-Reducing Bacteria Medium with Lactate

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1...............................................................................980.0mL

Solution 2.................................................................................10.0mL

Solution 3.................................................................................10.0mL

pH 7.4 ± 0.2 at 25°C

Solution 1:

Composition per 980.0mL:

Sodium lactate (70% solution)...................................................... 3.5g

MgSO4·7H2O ................................................................................ 2.0g

NH4Cl ........................................................................................... 1.0g

Na2SO4.......................................................................................... 1.0g

Yeast extract.................................................................................. 1.0g

K2HPO4......................................................................................... 0.5g

CaCl2·2H2O................................................................................... 0.1g

Preparation of Solution 1: Add components to distilled/deionized

water and bring volume to 980.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C.

Solution 2:

Composition per 10.0mL:

FeSO4·7H2O.................................................................................. 0.5g

Preparation of Solution 2: Add FeSO4·7H2O to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C.

Solution 3:

Composition per 10.0mL:

Ascorbic acid ................................................................................ 0.1g

Sodium thioglycolate .................................................................... 0.1g

Preparation of Solution 3: Add components to distilled/deionized

water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Aseptically combine 980.0mL of cooled,

sterile solution 1, 10.0mL of cooled, sterile solution 2, and 10.0mL of

cooled, sterile solution 3. Mix thoroughly. Aseptically distribute into

sterile tubes or flasks.

Use: For the enrichment and isolation of sulfate-reducing bacteria.