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Styrene Mineral Salts Broth

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

(NH4)2SO4 .................................................................................... 2.0g

K2HPO4 ...................................................................................... 1.55g

NaH2PO·2H2O............................................................................ 0.85g

MgCl2·6H2O ................................................................................. 0.1g

EDTA....................................................................................... 10.0mg

FeSO4·7H2O .............................................................................. 5.0mg

ZnSO4 ........................................................................................ 2.0mg

CaCl2·2H2O ............................................................................... 1.0mg

MnCl2·2H2O .............................................................................. 1.0mg

CoCl2·6H2O............................................................................... 0.4mg

CuSO4·5H2O.............................................................................. 0.2mg

Na2MoO4·2H2O......................................................................... 0.2mg

Styrene .......................................................................................0.1mL

Preparation of Medium: Add components, except styrene, to dis

tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Dis

tribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–

121°C. Under a fume hood, aseptically add styrene to screw-capped

tubes or flasks (5.0μl of styrene into 50.0mL of sterile mineral salts so

lution). Seal caps tightly.

Use: For the cultivation and maintenance of styrene-utilizing microor

ganisms.