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Styrene Mineral Salts Agar

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 20.0g

(NH4)2SO4 .................................................................................... 2.0g

K2HPO4 ...................................................................................... 1.55g

NaH2PO·2H2O............................................................................ 0.85g

MgCl2·6H2O ................................................................................. 0.1g

EDTA .......................................................................................10.0mg

FeSO4·7H2O ..............................................................................5.0mg

ZnSO4 ........................................................................................2.0mg

CaCl2·2H2O ...............................................................................1.0mg

MnCl2·2H2O ..............................................................................1.0mg

CoCl2·6H2O ...............................................................................0.4mg

CuSO4·5H2O..............................................................................0.2mg

Na2MoO4·2H2O.........................................................................0.2mg

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.

Place plates in a desiccator. Add to the desiccator an open bottle con

taining 10.0mL of dibutyl phthalate and 200.0μl of styrene.

Use: For the cultivation of styrene-utilizing microorganisms.