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Succinate Mineral Medium

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

MnSO4·7H2O.............................................................................. 62.0g

Succinate....................................................................................... 2.7g

K2HPO4·3H2O ............................................................................ 1.63g

NH4Cl ......................................................................................... 1.02g

KH2PO4....................................................................................... 0.39g

Trace elements solution .............................................................1.0mL

pH 6.8 ± 0.2 at 25°C

Trace Elements Solution:

Composition per 100.0mL:

Solution A................................................................................50.0mL

Solution B ................................................................................50.0mL

Preparation of Trace Elements Solution: Aseptically combine

50.0mL of sterile solution A with 50.0mL of sterile solution B.

Solution A:

Composition per liter:

Disodium EDTA ......................................................................... 0.25g

CaCl2·2H2O ................................................................................ 0.14g

FeCl2·4H2O................................................................................. 0.14g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C.

Solution B:

Composition per liter:

Disodium EDTA ......................................................................... 0.25g

H3BO3 ...................................................................................... 30.0mg

CoCl2·2H2O ............................................................................. 28.0mg

ZnCl2.......................................................................................... 5.0mg

MnCl2·4H2O .............................................................................. 4.0mg

NaMoO4·2H2O........................................................................... 3.0mg

NiCl2·6H2O.............................................................................. 1.65mg

CuCl2·2H2O ............................................................................... 0.7mg

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C.

Preparation of Medium: Add components, except trace elements

solution, to distilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Aseptical

ly add 1.0mL of sterile trace elements solution. Mix thoroughly. Asep

tically distribute into sterile tubes or flasks.

Use: For the cultivation of bacteria that can utilize succinate as a car

bon source.

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Succinate Mineral Medium