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Stygiolobus Medium

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

Stygiolobus Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sulfur flowers ............................................................................... 5.0g

(NH4)2SO4..................................................................................... 1.3g

KH2PO4....................................................................................... 0.28g

MgSO4·7H2O .............................................................................. 0.25g

Yeast extract.................................................................................. 0.2g

CaCl2·2H2O................................................................................. 0.07g

FeCl3·6H2O ................................................................................. 0.02g

Na2B4O7·10H2O.........................................................................4.5mg

MnCl2·4H2O...............................................................................1.8mg

Resazurin ...................................................................................0.5mg

ZnSO4·7H2O ............................................................................0.22mg

CuCl2·2H2O .............................................................................0.05mg

Na2MoO4·2H2O .......................................................................0.03mg

VOSO4·2H2O...........................................................................0.03mg

CoSO4 ......................................................................................0.01mg

pH 2.5 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 2.5 with

10N H2SO4. Distribute 20.0mL of medium into 100.0mL flasks or se

rum bottles. Autoclave for 15 min at 15 psi pressure–121°C. After in

oculation, pressurize bottles to 200KPa with 80%H2 + 20% CO2 .

Use: For the cultivation and maintenance of Stygiolobus azoricus.