培养基配方

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Stuart Leptospira Broth, Modified

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ............................................................................................ 1.93g

Na2HPO4 ..................................................................................... 0.66g

NH4Cl ......................................................................................... 0.34g

MgCl2·6H2O................................................................................ 0.19g

L-Asparagine ............................................................................... 0.13g

KH2PO4....................................................................................... 0.08g

Glycerol .....................................................................................5.0mL

Rabbit serum, inactivated at 56°C, 30 min ............................100.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add each component, except rabbit se

rum, to distilled/deionized water in separate flasks and bring each vol

ume to 100.0mL. Mix thoroughly. Combine the seven solutions, except

the rabbit serum. Mix thoroughly. Gently heat and bring to boiling. Au

toclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asep

tically add sterile rabbit serum. Mix thoroughly. Aseptically distribute

into sterile tubes or flasks.

Use: For the cultivation of Leptospira species.