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STTA Medium

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

STTA Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone........................................................................................ 20.0g

Glycerol ...................................................................................... 15.0g

Agar ............................................................................................ 13.0g

Yeast extract.................................................................................. 2.0g

K2HPO4......................................................................................... 1.0g

MgSO4·4H2O ................................................................................ 1.0g

Antibiotic solution ...................................................................10.0mL

Thallous acetate solution .........................................................10.0mL

pH 7.0 ± 0.2 at 25°C

Antibiotic Solution:

Composition per 10.0mL:

Streptomycin sulfate ..................................................................... 0.5g

Cycloheximide ............................................................................ 0.05g

Preparation of Antibiotic Solution: Add cycloheximide and

streptomycin sulfate to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Thallous Acetate Solution:

Composition per 10.0mL:

Thallous acetate .......................................................................... 0.05g

Preparation of Thallous Acetate Solution: Add thallous acetate

to distilled/deionized water and bring volume to 10.0mL. Mix thorough

ly. Filter sterilize.

Preparation of Medium: Add components, except antibiotic solu

tion and thallous acetate solution, to distilled/deionized water and

bring volume to 980.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–

50°C. Aseptically add sterile antibiotic solution and thallous acetate

solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into

sterile tubes.

Use: For the selective isolation and cultivation of Brochothrix ther

mosphacta.