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Stuart Medium Base

[所属分类:培养基配方] [发布时间:2021-8-30] [发布人:网站管理员2] [阅读次数:] [返回]

Stuart Medium Base

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1100.0mL:

NaCl.............................................................................................. 1.8g

Na2HPO4..................................................................................... 0.67g

MgCl2·6H2O ............................................................................... 0.41g

NH4Cl ......................................................................................... 0.27g

Asparagine .................................................................................. 0.13g

KH2PO4....................................................................................... 0.09g

Phenol Red.................................................................................. 0.01g

Glycerol .....................................................................................5.0mL

Leptospira enrichment...........................................................100.0mL

pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed powder from BD Di

agnostic Systems. Leptospira enrichment contains rabbit serum and

hemoglobin and is available from BD Diagnostic Systems.

Preparation of Medium: Add components, except glycerol and

Leptospira enrichment, to distilled/deionized water and bring volume

to 995.0mL. Mix thoroughly. Add glycerol. Mix thoroughly. Autoclave

for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically

add Leptospira enrichment. Mix thoroughly. Aseptically distribute into

sterile screw-capped tubes in 10.0mL volumes.

Use: For the cultivation of Leptospira species.