Ampicillin Dextrin Agar

[所属分类:培养基配方] [发布时间:2019-10-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ampicillin Dextrin Agar
Composition per liter:
Agar............................................................................................15.0g
Dextrin........................................................................................10.0g
NaCl..............................................................................................3.0g
Yeast extract..................................................................................2.0g
KCl................................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................0.2g
FeCl 3 ·4H 2 O...................................................................................0.1g
Selective supplement solution .................................................10.0mL
pH 8.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Selective Supplement Solution:
Composition per 10.0mL:
Sodium deoxycholate.............................................................100.0mg
Ampicillin................................................................................10.0mg
Preparation of Selective Supplement Solution: Add compo-
nents to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except selective sup-
plement solution and kanamycin solution, to distilled/deionized water
and bring volume to 990.0mL. Mix thoroughly. Autoclave for 15 min
at 15 psi pressure–121°C. Aseptically add 10.0mL of sterile selective
supplement solution. Mix thoroughly. Pour into Petri dishes or asepti-
cally distribute into sterile tubes.
Use: For the selective isolation and differentiation of Aeromonas spp.
from water samples.