Ampicillin Dextrin Agar with Vancomycin (ADA-V)

[所属分类:培养基配方] [发布时间:2019-10-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Ampicillin Dextrin Agar with Vancomycin
(ADA-V)
Composition per liter:
Agar ............................................................................................13.0g
Dextrin........................................................................................11.4g
Tryptose ........................................................................................5.0g
NaCl..............................................................................................3.0g
KCl................................................................................................2.0g
Yeast extract..................................................................................2.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Bromothymol Blue .....................................................................0.08g
FeCl 3 ·6H 2 O.................................................................................0.06g
Sodium deoxycholate.................................................................1.0mg
Ampicillin solution..................................................................10.0mL
Vancomycin solution................................................................10.0mL
pH 8.0 ± 0.2 at 25°C
Ampicillin Solution:
Composition per 10.0mL:
Ampicillin................................................................................10.0mg
Preparation of Ampicillin Solution:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Vancomycin Solution:
Composition per 10.0mL:
Vancomycin ...............................................................................2.0mg
Preparation of Vancomycin Solution:  Add components to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except sodium deoxy-
cholate, ampicillin solution, and vancomycin solution, to distilled/de-
ionized water and bring volume to 980.0mL. Mix thoroughly. Adjust
pH to 8.0. Add sodium deoxycholate. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 50°C. Aseptically add ampicillin and vanco-
mycin solutions. Mix thoroughly. Pour into Petri dishes or aseptically
distribute into sterile tubes.
Use: For the selective isolation and differentiation of Aeromonas spp.
from water samples.