Amphibacillus Medium

[所属分类:培养基配方] [发布时间:2019-10-9] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Amphibacillus Medium
Composition per liter:
Agar............................................................................................15.0g
Glucose.......................................................................................10.0g
Yeast extract..................................................................................3.0g
NH 4 NO 3 ........................................................................................2.0g
K 2 HPO 4 .........................................................................................1.0g
Polypeptone™ (pancreatic digest of casein and
peptic digest of animal tissue)................................................0.3g
MgSO 4 ·7H 2 O................................................................................0.2g
CaCl 2 ·2H 2 O ..................................................................................0.1g
FeSO 4 ·7H 2 O...............................................................................5.0mg
MnSO 4 ·7H 2 O.............................................................................5.0mg
pH 9.7 ± 0.2 at 25°C
Sodium Sesquicarbonate Solution:
Composition per 100.0mL:
Na 2 CO 3 , anhydrous.....................................................................10.6g
NaHCO 3 ......................................................................................8.42g
Preparation of Sodium Sesquicarbonate Solution : Add com-
ponents to distilled/deionized water and bring volume to 100.0mL.
Mix thoroughly. Filter sterilize. Warm to 50°–55°C.
Preparation of Medium: Add components, except sodium sesqui-
carbonate solution, to distilled/deionized water and bring volume to
900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave
for 15 min at 15 psi pressure–121°C. Cool to 50°–55°C. Aseptically
add sterile sodium sesquicarbonate solution. Mix thoroughly. Adjust
pH to 9.7. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Amphibacillus xylanus.