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Streptomycete Medium

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glycerol ........................................................................................ 5.0g

Agar .............................................................................................. 4.0g

NaCl .............................................................................................. 2.0g

KNO3 ............................................................................................ 1.0g

Na2HPO4·2H2O......................................................................... 0.534g

MgSO4·7H2O ................................................................................ 0.5g

KH2PO4..................................................................................... 0.272g

Trace elements solution .............................................................1.0mL

pH 6.8 ± 0.2 at 25°C

Trace Elements Solution:

Composition per 100.0mL:

FeSO4·7H2O.................................................................................. 0.1g

MnCl2·4H2O.................................................................................. 0.1g

ZnSO4·7H2O ................................................................................. 0.1g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 100.0mL. Mix thorough

ly.

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes in 1.0mL volumes. Autoclave for 15

min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of streptomycetes based on

their reduction of nitrate to nitrite. Bacteria that reduce nitrate to nitrite

form a red color after the addition of Griess-Ilosvay reagent.