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Streptomycete Medium

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution B ..............................................................................500.0mL

Solution A ..............................................................................400.0mL

Solution C ..............................................................................100.0mL

Solution A:

Composition per 400.0mL:

Glucose ....................................................................................... 20.0g

Agar .............................................................................................. 4.0g

Yeast extract.................................................................................. 1.2g

MgSO4·7H2O .............................................................................. 0.25g

Bromcresol Purple .................................................................... 0.012g

Preparation of Solution A: Add components to distilled/deionized

water and bring volume to 400.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution B:

Composition per 500.0mL:

Na2HPO4·2H2O......................................................................534.0mg

KH2PO4..................................................................................272.0mg

Preparation of Solution B: Add components to distilled/deionized

water and bring volume to 500.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 45°–50°C.

Solution C:

Composition per 100.0mL:

CaCO3 ........................................................................................... 1.0g

Preparation of Solution C: Add CaCO3 to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly.

Preparation of Medium: Distribute solution C into test tubes in

0.2mL volumes. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Combine cooled, sterile solution A and cooled, sterile so

lution B. Mix thoroughly. Add 1.8mL of solution A-B to each test tube

containing sterile solution C. Mix thoroughly to distribute the CaCO3.

Cool tubes rapidly in an ice-water bath.

Use: For the cultivation and differentiation of streptomycetes based on

their formation of organic acids. Bacteria that form organic acids turn

the medium yellow and dissolve the CaCO3.