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Streptomycete Medium

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 12.0g

NaCl.............................................................................................. 5.0g

Na2HPO4·2H2O........................................................................... 1.98g

KH2PO4....................................................................................... 1.51g

Glucose ......................................................................................... 1.0g

Pancreatic digest of casein............................................................ 1.0g

MgSO4·7H2O................................................................................ 0.5g

Phenol Red................................................................................ 0.012g

Urea solution..........................................................................100.0mL

pH 6.8 ± 0.2 at 25°C

Urea Solution:

Composition per 100.0mL:

Urea............................................................................................. 20.0g

Preparation of Urea Solution: Add urea to distilled/deionized wa

ter and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except urea solution, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add 100.0mL of sterile

urea solution. Mix thoroughly. Aseptically distribute into sterile tubes.

Allow tubes to cool in a slanted position.

Use: For the cultivation and differentiation of streptomycetes based on

their ability to produce urease.