培养基配方

您现在的位置：网站首页 >> 培养基配方

Streptomycete Antibiotic Activity Medium

[所属分类:培养基配方] [发布时间:2021-8-27] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................ 15.0g

D-Glucose.................................................................................... 15.0g

Soybean meal.............................................................................. 15.0g

NaCl.............................................................................................. 5.0g

Yeast extract.................................................................................. 1.0g

CaCO3 ........................................................................................... 1.0g

Glycerol .....................................................................................2.5mL

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring

to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15

psi pressure–121°C. Pour into sterile Petri dishes.

Use: For the the cultivation and determination of antibiotic production

of Streptomyces species by the streak method. Bacillus subtilis NRRL

B-765, Sarcina lutea NRRL B-1018, Escherichia coli NRRL B-766,

Saccharomyces pasteurianus NRRL Y-139, Candida albicans NRRL

Y-477, and Mucor ramannianus NRRL 1839 are used as test organ

isms.