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Spiroplasma Medium with 25 mg/L of Phenol Red

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

Spiroplasma Medium

with 25 mg/L of Phenol Red

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................ 80.0g

Beef heart, solids from infusion.................................................. 34.7g

Peptone.......................................................................................... 6.9g

NaCl .............................................................................................. 3.5g

Phenol Red...............................................................................25.0mg

Horse serum, heat inactivated................................................100.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add heat-inactivated

horse serum. Mix thoroughly. Aseptically distribute into sterile tubes

or flasks.

Use: For the cultivation and maintenance of Spiroplasma floricola.