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Spiroplasma Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

Spiroplasma Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sorbitol........................................................................................ 70.0g

Pancreatic digest of casein............................................................ 7.0g

Yeast extract.................................................................................. 5.0g

NaCl .............................................................................................. 5.0g

Beef extract ................................................................................... 3.0g

Yeast extract.................................................................................. 3.0g

Beef heart, solids from infusion.................................................... 2.0g

Fructose......................................................................................... 1.0g

Glucose ......................................................................................... 1.0g

Phenol Red...............................................................................20.0mg

Horse serum ...........................................................................100.0mL

pH 7.8 ± 0.2 at 25°C

Preparation of Medium: Add components to distilled/deionized

water and bring volume to 900.0mL. Mix thoroughly. Gently heat and

bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 50°–55°C. Aseptically add 100.0mL of sterile horse serum. Mix

thoroughly.

Use: For the cultivation of Spiroplasma citri.