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Spizizen Potato Agar

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Potatoes..................................................................................... 200.0g

Agar ............................................................................................ 15.0g

MnSO4 .......................................................................................5.0mg

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Peel and dice potatoes. Add potatoes to

1.0L of tap water. Gently heat and bring to boiling. Continue boiling

for 30 min. Filter through cheesecloth. Add MnSO4 to filtrate and bring

volume to 1.0L with tap water. Mix thoroughly. Adjust pH to 6.8. Add

agar. Gently heat and bring to boiling. Distribute into tubes or flasks.

Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri

dishes or leave in tubes.

Use: For the cultivation and maintenance of Bacillus amyloliquefa

ciens.