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Spiroplasma Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

Spiroplasma Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose........................................................................................ 80.0g

Beef heart, solids from infusion.................................................. 34.7g

Peptone ......................................................................................... 6.9g

NaCl.............................................................................................. 3.5g

Horse serum, heat inactivated................................................100.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Add components, except horse serum, to

distilled/deionized water and bring volume to 900.0mL. Mix thorough

ly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Asep

tically add horse serum. Mix thoroughly. Aseptically distribute into

sterile tubes or flasks.

Use: For the cultivation and maintenance of Spiroplasma species.