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Spirochaeta litoralis Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein............................................................ 3.0g

NaCl.............................................................................................. 2.0g

Yeast extract.................................................................................. 0.5g

Glucose solution ........................................................................2.0mL

Potasssium phosphate

buffer (1M, pH 7.4) .............................................................2.0mL

Sulfide solution..........................................................................0.5mL

Salts solution..............................................................................0.2mL

pH 7.3 ± 0.2 at 25°C

Glucose Solution:

Composition per 100.0mL:

D-Glucose.................................................................................... 25.0g

Preparation of Glucose Solution: Add D-glucose to distilled/de

ionized water and bring volume to 100.0mL. Mix thoroughly. Filter

sterilize.

Sulfide Solution:

Composition per 100.0mL:

Na2S·9H2O.................................................................................. 10.0g

Preparation of Sulfide Solution: Add Na2S·9H2O to distilled/de

ionized water and bring volume to 100.0mL. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 25°C.

Salts Solution:

Composition per 100.0mL:

MgSO4·7H2O.............................................................................. 12.5g

CaCl2·2H2O ................................................................................ 3.75g

EDTA............................................................................................ 1.0g

FeSO4·7H2O.................................................................................. 0.5g

Trace elements solution ...........................................................25.0mL

Preparation of Salts Solution: Add components to distilled/deion

ized water and bring volume to 100.0mL. Mix thoroughly.

Trace Elements Solution:

Composition per 1800.0mL:

H3BO3 ........................................................................................... 5.5g

MnCl2·4H2O ................................................................................. 3.5g

AlCl3·6H2O................................................................................... 0.5g

CoCl2·6H2O .................................................................................. 0.5g

CuCl2·2H2O .................................................................................. 0.5g

NiCl2·6H2O................................................................................... 0.5g

ZnCl2............................................................................................. 0.5g

KI ................................................................................................ 0.25g

LiCl............................................................................................. 0.25g

Na2MoO4·2H2O .......................................................................... 0.25g

BaCl2·2H2O ................................................................................ 0.15g

SnCl2·2H2O................................................................................. 0.15g

NaVO3 ........................................................................................ 0.05g

Preparation of Trace Elements Solution: Add components to

distilled/deionized water and bring volume to 1800.0mL. Mix thor

oughly. Adjust pH to 3–4 with HCl.

Preparation of Medium: Add components, except glucose solu

tion and sulfide solution, to distilled/deionized water and bring volume

to 997.5mL. Mix thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Asepti

cally add sterile glucose solution and sulfide solution. Mix thoroughly.

Aseptically distribute into sterile tubes or flasks.

Use: For the isolation of Spirochaeta litoralis from marine habitats.

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Spirochaeta litoralis Medium