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Spirochaeta litoralis Medium

[所属分类:培养基配方] [发布时间:2021-8-23] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose ......................................................................................... 2.0g

Pancreatic digest of casein............................................................ 1.0g

Yeast extract.................................................................................. 1.0g

L-Cysteine ..................................................................................... 0.5g

Resazurin ...................................................................................1.0mg

Seawater.................................................................................750.0mL

Tris·HCl buffer (1.0M solution, pH 7.5) ..................................50.0mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: Prepare and dispense medium under

100% N2. Add components to distilled/deionized water and bring vol

ume to 1.0L. Mix thoroughly. Sparge with 100% N2. Adjust pH to 7.5

while continuing to sparge with 100% N2. Anaerobically distribute into

tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Final

pH of medium after autoclaving should be 7.2.

Use: For the cultivation and maintenance of Spirochaeta litoralis.