Saccharolytic Clostridia Medium

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Saccharolytic Clostridia Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium thioglycolate....................................................................1.0g
K 2 HPO 4 .........................................................................................0.8g
KH 2 PO 4 .........................................................................................0.2g
MgSO 4 ·7H 2 O................................................................................0.2g
NaCl..............................................................................................0.2g
Na 2 MoO 4 ·2H 2 O........................................................................0.025g
Yeast extract................................................................................0.01g
FeSO 4 ·7H 2 O................................................................................0.01g
MnSO 4 ·4H 2 O..............................................................................0.01g
CaCl2..........................................................................................0.01g
Carbohydrate solution............................................................100.0mL
Soil extract...............................................................................10.0mL
Trace elements solution.............................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Glucose or sucrose......................................................................10.0g
Preparation of Carbohydrate Solution: Add glucose or sucrose
to distilled/deionized water and bring volume to 100.0mL. Mix thor-
oughly. Filter sterilize.
Soil Extract:
Composition per 200.0mL:
Garden soil, neutral...................................................................100.0g
Preparation of Soil Extract: Add garden soil to 100.0mL of tap
water. Gently heat and bring to 130°C for 60 min. Cool to 45°C. Filter
through Whatman #1 filter paper. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 45°–50°C.
Trace Elements Solution:
Composition per liter:
Na 2 B 4 O 7 ·10H 2 O..........................................................................0.05g
CoNO 3 ·6H 2 O ..............................................................................0.05g
CdSO 4 ·2H 2 O...............................................................................0.05g
CuSO 4 ·5H 2 O...............................................................................0.05g
ZnSO 4 ·7H 2 O...............................................................................0.05g
MnSO 4 ·H 2 O................................................................................0.05g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except sodium thiogly-
colate and carbohydrate solution, to distilled/deionized water and bring
volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling.
Add sodium thioglycolate. Mix thoroughly. Distribute 9.5mL into test
tubes that contain inverted Durham tubes. Autoclave for 15 min at 15
psi pressure–121°C. Cool to 45°–50°C. Aseptically add 0.5mL of ster-
ile carbohydrate solution to each tube. Mix thoroughly.
Use: For the isolation of N 2 -fixing, saccharolytic Clostridium species.