Saccharolytic Clostridia Medium

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Saccharolytic Clostridia Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................6.0g
Sodium thioglycolate....................................................................0.5g
Carbohydrate solution............................................................100.0mL
Potassium phosphate (1M solution, pH 7.5)............................30.0mL
MgSO 4 (1M solution) ................................................................1.0mL
Solution M .................................................................................0.5mL
FeSO 4 solution...........................................................................0.2mL
pH 7.0–7.2 at 25°C
Carbohydrate Solution:
Composition per 100.0mL:
Glucose or sucrose......................................................................20.0g
Preparation of Carbohydrate Solution: Add carbohydrate to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Solution M:
Composition per liter:
CaCl 2 ...........................................................................................3.33g
MnCl 2 ·4H 2 O ...............................................................................1.98g
Na 2 MoO 4 ·2H 2 O..........................................................................1.21g
CoCl 2 ·6H 2 O................................................................................1.19g
Preparation of Solution M: Add components to distilled/deion-
ized water and bring volume to 1.0L. Mix thoroughly.
FeSO 4 ·7H 2 O Solution:
Composition per 10.0mL:
FeSO 4 ·7H 2 O................................................................................55.6g
H 2 SO 4 (0.1M solution).............................................................10.0mL
Preparation of FeSO 4 ·7H 2 O Solution: Add the FeSO 4 ·7H 2 O to
10.0mL of H 2 SO 4 solution. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except carbohydrate
solution, to distilled/deionized water and bring volume to 900.0mL.
Mix thoroughly. Gently heat and bring to boiling. Autoclave for 20 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile
carbohydrate solution. Mix thoroughly. Aseptically distribute into ster-
ile tubes.
Use: For the cultivation of saccharolytic Clostridium species.