Saccharomyces Medium

[所属分类:培养基配方] [发布时间:2021-8-10] [发布人:网站管理员2] [阅读次数:] [返回]

Saccharomyces Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Peptone........................................................................................20.0g
Agar ............................................................................................15.0g
Yeast extract................................................................................10.0g
Yeast nitrogen base without amino acids......................................6.7g
(NH 4 ) 2 SO 4 .....................................................................................5.0g
Proline........................................................................................4.0mg
Preparation of Medium: Add glucose, yeast nitrogen base without
amino acids, and proline to distilled/deionized water and bring volume
to 200.0mL. Mix thoroughly. Filter sterilize. Add peptone, yeast ex-
tract, (NH 4 ) 2 SO 4 , and agar to distilled/deionized water and bring vol-
ume to 800.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Asepti-
cally combine the two sterile solutions. Mix thoroughly. Pour into ster-
ile Petri dishes or aseptically distribute into sterile tubes.
Use: For the cultivation and maintenance of Saccharomyces cerevi-
siae.