ALOA Medium (Agar Listeria Ottavani & Agosti) (BAM M10a)

[所属分类:培养基配方] [发布时间:2019-9-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ALOA Medium
(Agar Listeria Ottavani & Agosti)
(BAM M10a)
Composition per liter:
Agar............................................................................................18.0g
Peptone .......................................................................................18.0g
LiCl.............................................................................................10.0g
Yeast extract................................................................................10.0g
Tryptone........................................................................................6.0g
NaCl..............................................................................................5.0g
Na 2 HPO 4 .......................................................................................2.5g
Na-pyruvate ..................................................................................2.0g
Glucose.........................................................................................2.0g
Mg-glycerophosphate...................................................................1.0g
MgSO 4 ..........................................................................................0.5g
5-Bromo4-chloro-indolyl-β- D -glucopyranoside.........................0.05g
Phosphatidylinositol solution...................................................50.0mL
Amphotericin B solution..........................................................10.0mL
Nalidixic acid solution...............................................................5.0mL
Ceftazidime solution..................................................................5.0mL
Polymyxin B solution................................................................5.0mL
pH 7.2 ± 0.2 at 25°C
Nalidixic Acid Solution:
Composition per 5.0mL:
Nalidixic acid..............................................................................0.02g
Preparation of Nalidixic Acid Solution: Add nalidixic acid to
distilled/deionized water and bring volume to 5.0mL. Mix thoroughly.
Filter sterilize.
Ceftazidime Solution:
Composition per 5.0mL:
Ceftazidime.................................................................................0.02g
Preparation of Ceftazidime Solution: Add ceftazidime to dis-
tilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Fil-
ter sterilize.
Amphotericin B Solution:
Composition per 10.0mL:
Amphotericin B ..........................................................................0.01g
Dimethylforamide......................................................................7.5mL
HCL, 1M....................................................................................2.5mL
Preparation of Amphotericin B Solution: Add amphotericin B
to 2.5mL of 1M HCl. Mix thoroughly. Add 7.5 mL of dimethlyfor-
amide. Mix thoroughly. Filter sterilize.
Polymyxin B Solution:
Composition per 5.0mL:
Polymyxin B........................................................................... 76700U
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 5.0mL. Mix thoroughly. Fil-
ter sterilize.
Phosphatidylinositol Solution:
Composition per 50.0mL:
L -α-phosphatidylinositol ..............................................................2.0g
Preparation of Phosphatidylinositol Solution: Add  L -α-phos-
photidylinositol to cold distilled/deionized water and bring volume to
50.0mL. Stir for 30 min so a homogeneous suspension is obtained. Au-
toclave for 15 min at 15 psi pressure–121°C. Cool to 48–50°C.
Preparation of Medium: Add components, except phosphati-
dylinositol solution, nalidixic acid solution, cetazidime solution, am-
photericin B solution, and polymyxin B solution, to distilled/deionized
water and bring volume to 920.0mL. Mix thoroughly. Adjust the pH to
7.2. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi
pressure–121°C. Cool to 45°–50°C. Aseptically add 50.0mL sterile
phosphatidylinositol solution, 5.0mL sterile nalidixic acid solution,
5.0mL sterile cetazidime solution, 10.0mL sterile amphotericin B solu-
tion, and 5.0mL sterile polymyxin B solution. Mix thoroughly. Pour
into Petri dishes or distribute into sterile tubes.
Use: For the isolaltion and cultivation of Listeria spp. For the isolation
and cultivation of Literia spp. according to ISO standard 11290.