ALP Basal Medium (Aerobic Low Peptone Basal Medium)

[所属分类:培养基配方] [发布时间:2019-9-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

ALP Basal Medium
(Aerobic Low Peptone Basal Medium)
Composition per liter:
Agar ............................................................................................15.0g
(NH 4 ) 2 SO 4 .....................................................................................1.0g
Pancreatic digest of casein............................................................0.5g
Yeast extract..................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
KCl................................................................................................0.2g
Phenol Red..................................................................................0.02g
Substrate solution.....................................................................50.0mL
pH 7.8 ± 0.2 at 25°C
Substrate Solution:
Composition per 50.0mL:
Substrate........................................................................................0.1g
Preparation of Substrate Solution: Add substrate to distilled/de-
ionized water and bring volume to 50.0mL. Use sugars, carbohydrates,
n-butanol, other alcohols, or any acidogenic carbon source. Mix thor-
oughly. Filter sterilize.
Preparation of Medium: Add components, except substrate solu-
tion, to distilled/deionized water and bring volume to 950.0mL. Mix
thoroughly. Gently heat and bring to boiling. Adjust pH to 7.8. Distrib-
ute into screw-capped tubes in 3.0mL volumes. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 0.15mL
of sterile substrate solution to each tube. Mix thoroughly. Allow tubes
to cool in a slanted position.
Use: For the cultivation and differentiation of microorganisms based
on their ability to utilize a variety of carbon sources such as carbohy-
drates, alcohols, and other acidogenic substrates.