Allisonella Medium (DSMZ Medium 1006)

[所属分类:培养基配方] [发布时间:2019-9-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Allisonella Medium
(DSMZ Medium 1006)
Composition per liter:
DL -Histidine..................................................................................7.8g
Na 2 CO 3 .........................................................................................4.0g
Yeast extract..................................................................................4.0g
Trypticase......................................................................................1.0g
(NH 4 ) 2 SO 4 ..............................................................................480.0mg
NaCl.......................................................................................480.0mg
K 2 HPO 4 ..................................................................................292.0mg
KH 2 PO 4 ..................................................................................292.0mg
MgSO 4 ·7H 2 O.........................................................................100.0mg
CaCl 2 ·2H 2 O..............................................................................64.0mg
Resazurin ...................................................................................1.0mg
Cysteine solution......................................................................10.0mL
Volatile fatty acid mixture..........................................................3.1mL
pH 6.0 ± 0.2 at 25°C
Cysteine Solution:
Composition per 10.0mL:
L -Cysteine·HCl·H 2 O .....................................................................0.5g
Preparation of Cysteine Solution:  Add  L -cysteine·HCl·H 2 O to
distilled/deionized water and bring volume to 10.0mL. Mix thorough-
ly. Sparge with 100% N 2 . Autoclave for 15 min at 15 psi pressure–
121°C.
Volatile Fatty Acid Mixture:
Composition per 7.75mL:
Acetic acid...............................................................................4.25mL
Propionic acid..........................................................................1.50mL
Butyric acid................................................................................1.0mL
DL -2-Methyl butyric acid.........................................................0.25mL
iso-Butyric acid........................................................................0.25mL
iso-Valeric acid........................................................................0.25mL
n-Valeric acid...........................................................................0.25mL
Preparation of Volatile Fatty Acid Mixture: Combine compo-
nents. Mix thoroughly.
Preparation of Medium: Add components, except cysteine solu-
tion, volatile fatty acid mixture, and bicarbonate, to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Boil for several minutes. Cool to room temperature while
sparging with 100% CO 2 . Add the solid Na 2 CO 3  and 3.1 mL volatile
fatty acid mixture and 10.0mL cysteine solution. Adjust the pH to 6.0.
Distribute into serum bottles or Hungate tubes under 100% CO 2 . Au-
toclave for 15 min at 15 psi pressure–121°C.
Use: For the maintenance or cultivation of Allisonella spp.