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Allen and Arnon Medium with Nitrate

[所属分类:培养基配方] [发布时间:2019-9-30] [发布人:] [阅读次数:] [返回]

山东拓普生物工程有限公司

Shandong Tuopu Biol-Engineering Co.,Ltd

Allen and Arnon Medium with Nitrate
Composition per 1000.25mL:
Noble agar...................................................................................10.0g
KNO 3 ........................................................................................0.253g
NaNO 3 ......................................................................................0.212g
Solution A................................................................................25.0mL
Solution B................................................................................6.25mL
Solution A:
Composition  per 2.0L :
MgSO 4 ·7H 2 O (4% solution)..................................................500.0mL
CaCl 2 ·2H 2 O (1.2% solution)..................................................500.0mL
NaCl (3.8% solution).............................................................500.0mL
Microelements stock solution................................................500.0mL
Preparation of Solution A: Prepare individual solutions and com-
bine.
Microelements Stock Solution:
Composition per 1090.0mL:
H 3 BO 3 ....................................................................................572.0mg
MnCl 2 ·4H 2 O...........................................................................360.0mg
ZnSO 4 ·7H 2 O............................................................................44.0mg
MoO 3 ........................................................................................36.0mg
CuSO 4 ·5H 2 O............................................................................15.8mg
CoCl 2 ·6H 2 O ...............................................................................8.0mg
NH 4 VO 3 .....................................................................................4.6mg
A & A FeEDTA solution .......................................................160.0mL
Preparation of Microelements Stock Solution: Add compo-
nents to distilled/deionized water and bring volume to 1090.0mL. Mix
well.
A & A FeEDTA Solution:
Composition per 550.0mL:
Disodium EDTA·2H 2 O...............................................................20.4g
FeSO 4 ·7H 2 O................................................................................13.7g
KOH..............................................................................................5.2g
Preparation of A & A FeEDTA Solution: Dissolve 5.2g of KOH
in 186.0mL of distilled/deionized water. Add 20.4g of disodium
EDTA·2H 2 O. Add 13.7g of FeSO4·7H2O to 364.0mL of distilled/deion-
ized water. Combine the EDTA solution with the FeSO 4 solution. Sparge
solution with filtered air until color changes. The pH is about 3.5.
Solution B:
Composition per 500.0mL:
K 2 HPO 4 .......................................................................................28.0g
Preparation of Solution B: Add K 2 HPO 4 to distilled/deionized
water and bring volume to 500.0mL.
Preparation of Medium: Add agar, KNO 3 , and NaNO 3 to distilled/
deionized water and bring volume to 969.0mL. Mix thoroughly. Gently
heat and bring to boiling. Add 25.0mL of solution A. Autoclave for 15
min at 15 psi pressure–121°C. Add 6.25mL of solution B aseptically
after sterilization.
Use: For the cultivation and maintenance of Anabaena species and
Nostoc species.

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