RV5 Medium (DSMZ Medium 1147)

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

RV5 Medium
(DSMZ Medium 1147)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl ...........................................................................................15.0g
DL -malic acid ...............................................................................4.0g
BICINE [N,N-bis(2-hydroxyethyl)glycine] buffer.....................1.63g
K 2 HPO 4 .........................................................................................1.0g
NH 4 Cl ........................................................................................0.52g
Yeast extract..................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
EDTA.......................................................................................10.0mg
CaCl 2 ·2H 2 O ...............................................................................7.5gm
Vitamin solution.......................................................................10.0mL
NaHCO 3 solution.....................................................................10.0mL
Trace elements solution.............................................................1.0mL
pH 7.5 ± 0.2 at 25°C
Vitamin Solution:
Composition per 10.0mL:
Thiamine-HCl·2H 2 O..................................................................1.0mg
Biotin.......................................................................................0.03mg
Vitamin B 12 ..............................................................................0.02mg
Preparation of Vitamin Solution:  Add components to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
NaHCO 3 Solution:
Composition per 20.0mL:
NaHCO 3 ........................................................................................2.5g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 20.0mL. Mix thoroughly. Filter ster-
ilize. Gas under 80% N 2  + 20% CO 2 .
Trace Elements Solution:
Composition per liter:
EDTA............................................................................................5.2g
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O...........................................................................190.0mg
Na 2 MoO 4 ·2H 2 O.....................................................................188.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
VoSO 4 ·2H 2 O............................................................................30.0mg
Ni 2 Cl 2 ·6H 2 O ............................................................................25.0mg
CuCl 2 ·2H 2 O.............................................................................17.0mg
H 3 BO 3 ........................................................................................6.0mg
Na 2 WO 4 ·2H 2 O...........................................................................2.0mg
NaHSeO 3 ...................................................................................2.0mg
Preparation of Trace Elements Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Adjust pH to 3.0. Mix
thoroughly.
Preparation of Medium: Add components, except bicarbonate so-
lution and vitamin solution, to distilled/deionized water and bring vol-
ume to 980.0mL. Mix thoroughly. Adjust pH to 9.5. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to room temperature. Aseptically add vitamin solution. Mix thorough-
ly. Adjust pH to 9.5. Aseptically distribute into culture vessels. Before
inoculation aseptically add bicarbonate solution.
Use: For the cultivation of Rhodobaca bogoriensis.