R2YE Medium

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

R2YE Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1062.2mL:

Thiostrepton.............................................................................50.0mg
Basal solution.........................................................................800.0mL
TES (N-tris[hydroxymethyl]methyl-2-amino–ethane-
sulfonic acid) buffer.........................................................100.0mL
CaCl 2 ·2H 2 O solution................................................................80.2mL
Yeast extract solution...............................................................50.0mL
L- Proline solution.....................................................................15.0mL
KH 2 PO 4 solution......................................................................10.0mL
NaOH solution...........................................................................5.0mL
Trace elements solution .............................................................2.0mL
pH 7.2 ± 0.2 at 25°C
Basal Solution:
Composition per 800.0mL:
Sucrose......................................................................................103.0g
MgCl 2 ·6H 2 O..............................................................................10.12g
D- Glucose....................................................................................10.0g
K 2 SO 4 ..........................................................................................0.25g
Casamino acids.............................................................................0.1g
Preparation of Basal Solution: Add components to distilled/de-
ionized water and bring volume to 800.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
TES Buffer:
Composition per liter:
TES (N-tris[hydroxymethyl]
methyl-2-amino–ethane-
sulfonic acid) buffer..............................................................57.3g
Preparation of TES Buffer: Add TES to distilled/deionized water
and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.2. Filter
sterilize.
CaCl 2 ·2H 2 O Solution:
Composition per 100.0mL:
CaCl 2 ·2H 2 O.................................................................................3.68g
Preparation of CaCl 2 ·2H 2 O Solution: Add the CaCl 2 ·2H 2 O to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
Yeast Extract Solution:
Composition per 100.0mL:
Yeast extract................................................................................10.0g
Preparation of Yeast Extract Solution: Add yeast extract to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
L - Proline Solution:
Composition per 100.0mL:
L- Proline......................................................................................20.0g
Preparation of  L- Proline Solution: Add the proline to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
KH 2 PO 4 Solution:
Composition per 100.0mL:
KH 2 PO 4 .........................................................................................0.5g
Preparation of KH 2 PO 4 Solution Solution: Add the KH 2 PO 4 to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Filter sterilize.
NaOH Solution:
Composition per 100.0mL:
NaOH..........................................................................................40.0g
Preparation of NaOH Solution: Add the NaOH to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize
Trace Elements Solution:
Composition per liter:
FeCl 3 ·6H 2 O...................................................................................0.2g
ZnCl 2 ...........................................................................................0.04g
CuCl 2 ·2H 2 O................................................................................0.01g
MnCl 2 ·4H 2 O ...............................................................................0.01g
Na 2 B 4 O 7 ·10H 2 O..........................................................................0.01g
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O..................................................................0.01g
Preparation of Trace Elements Solution: Add components to
distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Filter sterilize.
Preparation of Medium: To 800.0mL of cooled, sterile basal solu-
tion, aseptically add the remaining components. Mix thoroughly. Asep-
tically distribute into sterile tubes or flasks.
Use:  For the cultivation and maintenance of Streptomyces lividans.