Russell Double-Sugar Agar

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Russell Double-Sugar Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Proteose peptone No. 3...............................................................12.0g
Lactose........................................................................................10.0g
NaCl..............................................................................................5.0g
Beef extract...................................................................................1.0g
Glucose .........................................................................................1.0g
Phenol Red................................................................................0.025g
pH 7.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to
boiling. Distribute into tubes. Autoclave for 15 min at 15 psi pressure–
121°C. Allow tubes to cool in a slanted position.
Use: For the identification of Gram-negative enteric bacilli based on
their fermentation of glucose and lactose. Bacteria that ferment both
glucose and lactose produce a yellow slant and yellow butt. Bacteria
that ferment glucose but do not ferment lactose produce a red slant and
a yellow butt. Bacteria that ferment neither glucose nor lactose produce
an unchanged pink-orange color.