Ruminococcus pasteurii Medium

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Ruminococcus pasteurii Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaHCO 3 ........................................................................................2.5g
Sodium tartrate..............................................................................2.0g
NaCl..............................................................................................1.0g
KCl................................................................................................0.5g
MgCl 2 ·6H 2 O..................................................................................0.4g
Na 2 S·9H 2 O..................................................................................0.36g
NH 4 Cl .........................................................................................0.25g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O.................................................................................0.15g
Resazurin ...................................................................................1.0mg
NaHCO 3 solution.....................................................................10.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
Trace elements solution SL-7 ....................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-7:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O ................................................................................0.19g
MnCl 2 ·4H 2 O..................................................................................0.1g
ZnCl 2 ...........................................................................................0.07g
H 3 BO 3 .......................................................................................0.062g
Na 2 MoO 4 ·2H 2 O ........................................................................0.036g
NiCl 2 ·6H 2 O...............................................................................0.024g
CuCl 2 ·2H 2 O ..............................................................................0.017g
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-7: Add the
FeCl 2 ·4H 2 O to the HCl. Add distilled/deionized water and bring vol-
ume to 1.0L. Add remaining components. Mix thoroughly. Autoclave
for 15 min at 15 psi pressure–121°C under 100% N 2 . Cool to room
temperature.
NaHCO 3 Solution:
Composition per 10.0mL:
NaHCO 3 ........................................................................................2.5g
Preparation of NaHCO 3 Solution: Add the NaHCO 3 to distilled/
deionized water and bring volume to 10.0mL. Mix thoroughly. Filter
sterilize.
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O..................................................................................0.36g
Preparation of Na 2 S·9H 2 O Solution: Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C under 100% N 2 .
Preparation of Medium: Add components—except NaHCO 3 solu-
tion, Na 2 S·9H 2 O solution, and trace elements solution SL-7—to dis-
tilled/deionized water and bring volume to 999.0mL. Mix thoroughly.
Adjust pH to 7.2. Gently heat and bring to boiling under 80% N 2 + 20%
CO 2 . Distribute into tubes in 9.8mL volumes under 80% N 2 + 20%
CO 2 . Cool to 25°C. Aseptically add 0.1mL of sterile NaHCO 3 solution
and 0.01mL of sterile trace elements solution SL-7 to each tube. Mix
thoroughly. Immediately prior to inoculation, aseptically add 0.1mL of
sterile Na 2 S·9H 2 O solution to each tube.
Use: For the cultivation and maintenance of Ruminococcus pasteurii.