Ruminococcus pasteurii Medium

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Ruminococcus pasteurii Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..............................................................................................1.0g
KCl................................................................................................0.5g
MgCl 2 ·6H 2 O .................................................................................0.4g
NH 4 Cl .........................................................................................0.25g
KH 2 PO 4 .........................................................................................0.2g
CaCl 2 ·2H 2 O ................................................................................0.15g
Resazurin...................................................................................1.0mg
Biotin.........................................................................................0.4mg
2,3-Butanediol solution............................................................50.0mL
NaHCO 3 solution.....................................................................20.0mL
Na 2 S·9H 2 O solution.................................................................10.0mL
Trace elements solution SL-10..................................................1.0mL
pH 7.2 ± 0.2 at 25°C
Trace Elements Solution SL-10:
Composition per liter:
FeCl 2 ·4H 2 O...................................................................................1.5g
CoCl 2 ·6H 2 O...........................................................................190.0mg
MnCl 2 ·4H 2 O ..........................................................................100.0mg
ZnCl 2 ........................................................................................70.0mg
Na 2 MoO 4 ·2H 2 O.......................................................................36.0mg
NiCl 2 ·6H 2 O..............................................................................24.0mg
H 3 BO 3 ........................................................................................6.0mg
CuCl 2 ·2H 2 O...............................................................................2.0mg
HCl (25% solution)..................................................................10.0mL
Preparation of Trace Elements Solution SL-10: Prepare and
dispense under 80% N 2 + 20% CO 2 . Add FeCl 2 ·4H 2 O to 10.0mL of
HCl solution. Mix thoroughly. Add distilled/deionized water and bring
volume to 1.0L. Add remaining components. Mix thoroughly. Filter
sterilize. Gas under 80% N 2  + 20% CO 2 .
2,3-Butanediol Solution:
Composition per 50.0mL:
2,3-Butanediol ..............................................................................0.9g
Preparation of 2,3-Butanediol Solution:  Add 2,3-butanediol to
distilled/deionized water and bring volume to 50.0mL. Mix thorough-
ly. Filter sterilize. Gas under 80% N 2  + 20% CO 2 .
NaHCO 3 Solution:
Composition per 20.0mL:
NaHCO 3 ........................................................................................2.5g
Preparation of NaHCO 3 Solution:  Add NaHCO 3 to distilled/de-
ionized water and bring volume to 20.0mL. Mix thoroughly. Filter ster-
ilize. Gas under 80% N 2  + 20% CO 2 .
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O..................................................................................0.36g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Gas under 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Preparation of Medium: Prepare medium and dispense under 80%
N 2 + 20% CO 2 . Add components, except 2,3-butanediol solution,
NaHCO 3 solution, and Na 2 S·9H 2 O solution, to distilled/deionized water
and bring volume to 970.0mL. Mix thoroughly. Sparge with 80% N 2 +
20% CO 2 . Autoclave for 15 min at 15 psi pressure–121°C. Aseptically and
anaerobically add 50.0mL of sterile 2,3-butanediol solution, 20.0mL of
sterile NaHCO 3 solution, and 10.0mL of sterile Na 2 S·9H 2 O solution. Mix
thoroughly. Aseptically and anaerobically distribute into sterile tubes or
flasks.
Use: For the cultivation and maintenance of Ruminococcus pasteurii.