Ruminococcus albus Medium

[所属分类:培养基配方] [发布时间:2021-8-6] [发布人:网站管理员2] [阅读次数:] [返回]

Ruminococcus albus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1001.0mL:

Pancreatic digest of casein............................................................5.0g
Na 2 CO 3  .........................................................................................4.0g
Glucose.........................................................................................3.0g
Cellobiose.....................................................................................2.0g
Yeast extract..................................................................................2.0g
L -Cysteine·HCl .............................................................................0.5g
Resazurin ...................................................................................1.0mg
Mineral solution 1....................................................................40.0mL
Mineral solution 2....................................................................40.0mL
Fatty acid mixture......................................................................1.0mL
Mineral Solution 1:
Composition per 100.0mL:
K 2 HPO 4 .........................................................................................0.6g
Preparation of Mineral Solution 1: Add K 2 HPO 4 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly.
Mineral Solution 2:
Composition per 100.0mL:
(NH 4 ) 2 SO 4 ....................................................................................2.0g
NaCl..............................................................................................2.0g
KH 2 PO 4 .........................................................................................0.6g
MgSO 4 ·7H 2 O..............................................................................0.25g
CaCl 2 ·7H 2 O ................................................................................0.16g
Preparation of Mineral Solution 2:  Add components to distilled/
deionized water and bring volume to 100.0mL. Mix thoroughly.
Fatty Acid Mixture:
Composition per 100.0mL:
Isobutyric acid..........................................................................10.0mL
Isovaleric acid..........................................................................10.0mL
2-Methylbutyric acid................................................................10.0mL
Preparation of Fatty Acid Mixture : Add components to distilled/
deionized water and bring volume to 100.0mL. Sparge with 100%
CO 2 .
Preparation of Medium: Add components, except Na 2 CO 3  ,  L -
cysteine·HCl, and fatty acid mixture, to distilled/deionized water and
bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boil-
ing. Continue boiling for 5 min. Cool to room temperature while sparg-
ing with 100% CO 2 . Add Na 2 CO 3  ,  L -cysteine·HCl, and fatty acid
mixture. Adjust pH to 7.0. Anaerobically distribute into tubes or flasks
under 100% N 2 . Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation and maintenance of Ruminococcus albus.