Rhodopseudomonas julia Medium

[所属分类:培养基配方] [发布时间:2021-8-4] [发布人:网站管理员2] [阅读次数:] [返回]

Rhodopseudomonas julia Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaHCO 3 ........................................................................................3.0g
NaCl..............................................................................................1.0g
KH 2 PO 4 .........................................................................................1.0g
NH 4 Cl ...........................................................................................1.0g
Sodium acetate..............................................................................1.0g
Na 2 SO 4 ..........................................................................................0.7g
MgCl 2 ·6H 2 O .................................................................................0.5g
Sodium ascorbate..........................................................................0.5g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Yeast extract..................................................................................0.1g
Na 2 S·9H 2 O solution.................................................................10.0mL
SLA trace elements solution......................................................1.0mL
VA vitamin solution...................................................................1.0mL
pH 6.9–7.0 at 25°C
Na 2 S·9H 2 O Solution:
Composition per 10.0mL:
Na 2 S·9H 2 O................................................................................0.156g
Preparation of Na 2 S·9H 2 O Solution:  Add Na 2 S·9H 2 O to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Autoclave for 15 min at 15 psi pressure–121°C. Before use, neutralize
to pH 7.0 with sterile HCl.
SLA Trace Elements Solution:
Composition per liter:
FeC1 2 ·4H 2 O..................................................................................1.8g
H 3 BO 3 ...........................................................................................0.5g
CoCl 2 ·6H 2 O................................................................................0.25g
ZnCl 2 .............................................................................................0.1g
MnCl 2 ·4H 2 O.............................................................................70.0mg
Na 2 MoO 4 ·2H 2 O .......................................................................30.0mg
CuCl 2 ·2H 2 O .............................................................................10.0mg
Na 2 SeO 3 ·5H 2 O.........................................................................10.0mg
NiCl 2 ·6H 2 O..............................................................................10.0mg
Preparation of SLA Trace Elements Solution: Add compo-
nents to distilled/deionized water and bring volume to 1.0L. Mix thor-
oughly. Adjust pH to 2.0–3.0.
VA Vitamin Solution:
Composition per liter:
Nicotinamide.............................................................................0.175g
Thiamine·HCl .............................................................................0.15g
p-Aminobenzoic acid....................................................................0.1g
Biotin .......................................................................................50.0mg
Calcium  D -(+)-pantothenate.....................................................50.0mg
Pyridoxine·2HCl......................................................................50.0mg
Cyanocobalamin ......................................................................25.0mg
Preparation of VA Vitamin Solution: Add components to dis-
tilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except Na 2 S·9H 2 O so-
lution, to distilled/deionized water and bring volume to 990.0mL. Mix
thoroughly. Adjust pH to 6.9–7.0. Autoclave for 15 min at 15 psi pres-
sure–121°C. Aseptically add 10.0mL of sterile Na 2 S·9H 2 O solution.
Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Rhodopseudomonas julia.